MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

产品名称

MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)

1592

3522

          质量

溶剂体积

浓度

体内研究（动物模型）：

动物模型（Animal Model）：C57BL/6J WT and Aqp9-/- mice

配制方法（Formulation）：MPP+ (7.5 μg dissolved in saline)

实验方法（Assay）：C57BL/6J WT (n = 34) and Aqp9-/- mice (n = 29) were deeply anesthetized with zoletil mixture, Rompunand Fentanyl and then subjected tostereotaxic intrastriatal injections of MPP+ (7.5 μg dissolved in saline) or saline. 1 μl MPP+ solution or saline was injected into the striatum 0.6 mm anterior to Bregma, 2.2 mm laterally and 3.2 mm ventrally at 12 μl/hr using a syringe pump. MPP+ was protected against light during the procedure. The injector was left for 5 minutes to allow diffusion before suture.

体外研究（细胞实验）：

细胞类型（Cell types）：PC12 cells

实验方法（Assay）：The in vitro PD model was established by treatment of PC12 cells with250 μM MPP+ for approximately 24 hours.Different concentrations of RvD1 (50, 100, 200 nM) were administrated two hours prior to MPP+ treatment, afterwards, PC12 cells were used for following experiments.

体内研究（动物模型）：

动物模型（Animal Model）：Zebrafish larvae

配制方法（Formulation）：Both MPP+ and PBA were dissolved in the system water.

实验方法（Assay）：Zebrafish larvae were incubated between 1 and 5 dpf in the presence or absence of 500 μM MPP+ at a total volume of 25 ml system water. The drug solutions were freshly prepared and replaced between 11:00 and 12:00 am at 2, 3, and 4 dpf.Deceased larvae were removed daily, but no differences in mortality were observed between MPP+ exposed larvae and untreated larvae.