描述
Alkali-Cation™ 酵母转化试剂盒易于使用、稳健,其可扩展性使其成为两种混合筛选所需的高效转化的首选方法。
应用说明
Alkali-Cation™ 酵母转化试剂盒提供了一种有效的酵母转化方法,并已成功用于将线性 DNA 转化为毕赤酵母以进行蛋白质表达研究。
使用声明
除非另有说明,MP Biomedical 的产品仅供研究或进一步制造使用,不能直接供人类使用。如需更多信息,请联系我们的客户服务部门。
关键应用
酵母细胞的转化
SKU | 112200200 |
Alternate Names | High Efficiency Yeast Transformation Kit |
Application Notes | Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies. |
Base Catalog Number | 2200200 |
Components | Sterile TE Buffer pH 7.5 |Lithium/Cesium Acetate |Carrier DNA 10 mg/mL Sterile |Sterile Solution Histamine |TE/Cation MIXX |PEG |Sterile Solution SOS |
Format | All necessary reagents |
Pack Size | 250 Preps |
Personal Protective Equipment | Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves. Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields. Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
Recommended Alternate | EZ-Yeast™ Transformation Kit |
Safety Symbol | GHS07 |GHS08 |
Sample Type | Overnight yeast culture or colonies from plates |
Sample Volume | 500 µL or 3-4 mm colony |
Unit Definition | ≥ 104 transformants/µg plasmid DNA |
Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |