【温馨提示I】：见碱性磷酸酶产品专题，了解更多相关产品资料；
【温馨提示II】：见碱性磷酸酶染色试剂盒产品；
【温馨提示III】：见碱性磷酸酶常用生化底物及试剂盒产品；
产品关键词：
Phosphatase, Alkaline碱性磷酸酶；ALP/AKP；对硝基苯酚磷酸盐（pNPP）；偶氮偶联法；显色法（定量）；染色法（定性）；
基本介绍：
► 碱性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一种含两个相同亚基的二聚体，也是一种约含12%碳水化合物（6%己糖和6%其他中性糖）的糖蛋白，每个ALP分子含4个锌离子和4个二硫键。镁离子存在情况下，ALP可达到最高酶活。碱性条件下，ALP能催化磷酸单酯水解，比如对硝基苯酚磷酸盐（pNPP）、磷酸苯酯、葡萄糖-6-磷酸、葡萄糖-1-磷酸、果糖-6-磷酸、腺苷-5-磷酸等。
► 常见的碱性磷酸酶ALP包括肠道碱性磷酸酶（如小牛肠来源，calf intestinal）、胎盘碱性磷酸酶（placental alkaline phosphatase）、非组织特异性碱性磷酸酶（tissue-nonspecific isozyme, alkaline phosphatase）等。
► ALP常用来偶联抗体和其他蛋白，从而满足ELISA、WB和组织化学检测上的应用。通常用来蛋白的去磷酸化实验，比如酪蛋白和核酸等。当需要高检测灵敏度时也能用来蛋白标记。还可用在DNA或RNA 的5′-末端去磷酸以防止自连。经碱性磷酸酶的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。
研究意义：
碱性磷酸酶（ALP）活性的变化与一系列生理和病理事件紧密关联，比如骨骼发育、骨骼相关疾病、妊娠相关疾病和炎症性肠病（inflammatory bowel diseases）等。正因如此，ALP被用作衡量生理特征或疾病的参照指标。另外，ALP活性检测在环境监测、食品质量控制等方面也呈现重要作用。
► 干细胞如诱导多潜能干细胞iPS，ALP活性很高，则ALP常被用作iPS诱导成功的标志；
► 分化的结肠癌细胞内ALP活性显著提高，则ALP常被当做结肠癌细胞分化程度的指标（定性/定量）；
► 血清中ALP水平提高，被称作高碱性磷酸酶血症，此症状被认为和恶性胆管阻塞、原发性胆管硬化、原发性硬化胆管炎、肝淋巴瘤和肝肉瘤等肝胆疾病密切相关；
► ALP是成骨细胞的副产物，血清中ALP活性升高和骨头发育密切关联；
► 血清ALP活性过低也与一些疾病相关；
► 儿童和孕妇血清内ALP活性较常人高一些；血清中ALP活性范围在20-140U/L。
检测原理（碱性磷酸酶活性）：
对硝基苯酚磷酸盐（pNPP）是一种常用的碱性磷酸酶（ALP）显色底物，碱性条件下能被ALP催化生成对硝基酚（para-nitrophenol，p-nitrophenol），呈黄色产物，可在400-415nm检测到吸光度。产物黄色越深，代表ALP活性越高，反之则酶活性越低。则通过比色分析可定量检测ALP水平。
反应式如下：
检测对象：
细胞或组织裂解液或匀浆液、血清、血浆、尿液等样品内源性碱性磷酸酶活性。
订购信息I：Anaspec国原装进口，>几十篇文献引用，高口碑产品。
| 品牌 |
名称 |
货号 |
规格 |
目录价（元） |
| Anaspec |
SensoLyte® pNPP Alkaline Phosphatase Assay Kit *Colorimetric* |
AS-72146 |
500 assays |
2422 |
试剂盒组分：
| Component |
Description |
Quantity |
| Component A |
pNPP, colorimetric alkaline phosphatase substrate |
25 mL |
| Component B |
10X Assay buffer |
50 mL |
| Component C |
Stop solution |
25 mL |
| Component D |
Triton-X-100 |
500μl |
| Component E |
Alkaline Phosphatase Standard, Calf Intestine |
10μg /mL, 50μl |
AS-72146 SensoLyte pNPP Kit Datasheet
订购信息II：国产碱性磷酸酶检测试剂盒，在保证优秀质量的情况下，提供更有竞争力的价格，欢迎选购。
| 品牌 |
名称 |
货号 |
规格 |
目录价（元） |
| MKbio |
Alkaline Phosphatase Assay Kit (Colorimetric) 碱性磷酸酶检测试剂盒（显 色法） |
MP7501-100T |
100T |
495 |
试剂盒组分：
| Component |
Description |
Quantity |
| Component A |
pNPP显色底物 |
2管 |
| Component B |
检测缓冲液 |
15 mL |
| Component C |
终止液 |
12 mL |
| Component D |
对硝基苯酚溶液（10mM） |
100μl |
引用文献（Anaspec，AS-72146）
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— —Written/Edited by V. Shallan【版权归集奇生物/MKBio懋康所有】
